Template:See introduction Template:Genetics sidebar Template:Biochemistry sidebar Genetics is the study of genes, genetic variation, and heredity in living organisms. It is generally considered a field of biology, but intersects frequently with many other life sciences and is strongly linked with the study of information systems.
The father of genetics is Gregor Mendel, a late 19th-century scientist and Augustinian friar. Mendel studied "trait inheritance", patterns in the way traits are handed down from parents to offspring. He observed that organisms (pea plants) inherit traits by way of discrete "units of inheritance". This term, still used today, is a somewhat ambiguous definition of what is referred to as a gene.
Trait inheritance and molecular inheritance mechanisms of genes are still primary principles of genetics in the 21st century, but modern genetics has expanded beyond inheritance to studying the function and behavior of genes. Gene structure and function, variation, and distribution are studied within the context of the cell, the organism (e.g. dominance), and within the context of a population. Genetics has given rise to a number of subfields, including epigenetics and population genetics. Organisms studied within the broad field span the domains of life (archaea, bacteria, and eukarya).
Genetic processes work in combination with an organism's environment and experiences to influence development and behavior, often referred to as nature versus nurture. The intracellular or extracellular environment of a cell or organism may switch gene transcription on or off. A classic example is two seeds of genetically identical corn, one placed in a temperate climate and one in an arid climate. While the average height of the two corn stalks may be genetically determined to be equal, the one in the arid climate only grows to half the height of the one in the temperate climate due to lack of water and nutrients in its environment.
The word genetics stems from the ancient Greek ="grc" xml:lang="grc" >γενετικός</span> ="grc-Latn" xml:lang="grc-Latn" >genetikos</span> meaning "genitive"/"generative", which in turn derives from ="grc" xml:lang="grc" >γένεσις</span> ="grc-Latn" xml:lang="grc-Latn" >genesis</span> meaning "origin".
- Main article: History of genetics
The observation that living things inherit traits from their parents has been used since prehistoric times to improve crop plants and animals through selective breeding. The modern science of genetics, seeking to understand this process, began with the work of the Augustinian friar Gregor Mendel in the mid-19th century.
Prior to Mendel, Imre Festetics, a Hungarian noble, who lived in Kőszeg before Mendel, was the first who used the word "genetics." He described several rules of genetic inheritance in his work The genetic law of the Nature (Die genetische Gesätze der Natur, 1819). His second law is the same as what Mendel published. In his third law, he developed the basic principles of mutation (he can be considered a forerunner of Hugo de Vries).
Other theories of inheritance preceded Mendel's work. A popular theory during the 19th century, and implied by Charles Darwin's 1859 On the Origin of Species, was blending inheritance: the idea that individuals inherit a smooth blend of traits from their parents. Mendel's work provided examples where traits were definitely not blended after hybridization, showing that traits are produced by combinations of distinct genes rather than a continuous blend. Blending of traits in the progeny is now explained by the action of multiple genes with quantitative effects. Another theory that had some support at that time was the inheritance of acquired characteristics: the belief that individuals inherit traits strengthened by their parents. This theory (commonly associated with Jean-Baptiste Lamarck) is now known to be wrong—the experiences of individuals do not affect the genes they pass to their children, although evidence in the field of epigenetics has revived some aspects of Lamarck's theory. Other theories included the pangenesis of Charles Darwin (which had both acquired and inherited aspects) and Francis Galton's reformulation of pangenesis as both particulate and inherited.
Mendelian and classical geneticsEdit
Modern genetics started with Mendel's studies of the nature of inheritance in plants. In his paper "Versuche über Pflanzenhybriden" ("Experiments on Plant Hybridization"), presented in 1865 to the Naturforschender Verein (Society for Research in Nature) in Brünn, Mendel traced the inheritance patterns of certain traits in pea plants and described them mathematically. Although this pattern of inheritance could only be observed for a few traits, Mendel's work suggested that heredity was particulate, not acquired, and that the inheritance patterns of many traits could be explained through simple rules and ratios.
The importance of Mendel's work did not gain wide understanding until 1900, after his death, when Hugo de Vries and other scientists rediscovered his research. William Bateson, a proponent of Mendel's work, coined the word genetics in 1905 (the adjective genetic, derived from the Greek word genesis—γένεσις, "origin", predates the noun and was first used in a biological sense in 1860). Bateson both acted as a mentor and was aided significantly by the work of female scientists from Newnham College at Cambridge, specifically the work of Becky Saunders, Nora Darwin Barlow, and Muriel Wheldale Onslow. Bateson popularized the usage of the word genetics to describe the study of inheritance in his inaugural address to the Third International Conference on Plant Hybridization in London in 1906.
After the rediscovery of Mendel's work, scientists tried to determine which molecules in the cell were responsible for inheritance. In 1911, Thomas Hunt Morgan argued that genes are on chromosomes, based on observations of a sex-linked white eye mutation in fruit flies. In 1913, his student Alfred Sturtevant used the phenomenon of genetic linkage to show that genes are arranged linearly on the chromosome.
Although genes were known to exist on chromosomes, chromosomes are composed of both protein and DNA, and scientists did not know which of the two is responsible for inheritance. In 1928, Frederick Griffith discovered the phenomenon of transformation (see Griffith's experiment): dead bacteria could transfer genetic material to "transform" other still-living bacteria. Sixteen years later, in 1944, the Avery–MacLeod–McCarty experiment identified DNA as the molecule responsible for transformation. The role of the nucleus as the repository of genetic information in eukaryotes had been established by Hämmerling in 1943 in his work on the single celled alga Acetabularia. The Hershey–Chase experiment in 1952 confirmed that DNA (rather than protein) is the genetic material of the viruses that infect bacteria, providing further evidence that DNA is the molecule responsible for inheritance.
James Watson and Francis Crick determined the structure of DNA in 1953, using the X-ray crystallography work of Rosalind Franklin and Maurice Wilkins that indicated DNA has a helical structure (i.e., shaped like a corkscrew). Their double-helix model had two strands of DNA with the nucleotides pointing inward, each matching a complementary nucleotide on the other strand to form what look like rungs on a twisted ladder. This structure showed that genetic information exists in the sequence of nucleotides on each strand of DNA. The structure also suggested a simple method for replication: if the strands are separated, new partner strands can be reconstructed for each based on the sequence of the old strand. This property is what gives DNA its semi-conservative nature where one strand of new DNA is from an original parent strand.
Although the structure of DNA showed how inheritance works, it was still not known how DNA influences the behavior of cells. In the following years, scientists tried to understand how DNA controls the process of protein production. It was discovered that the cell uses DNA as a template to create matching messenger RNA, molecules with nucleotides very similar to DNA. The nucleotide sequence of a messenger RNA is used to create an amino acid sequence in protein; this translation between nucleotide sequences and amino acid sequences is known as the genetic code.
With the newfound molecular understanding of inheritance came an explosion of research. A notable theory arose from Tomoko Ohta in 1973 with her amendment to the neutral theory of molecular evolution through publishing the nearly neutral theory of molecular evolution. In this theory, Ohta stressed the importance of natural selection and the environment to the rate at which genetic evolution occurs. One important development was chain-termination DNA sequencing in 1977 by Frederick Sanger. This technology allows scientists to read the nucleotide sequence of a DNA molecule. In 1983, Kary Banks Mullis developed the polymerase chain reaction, providing a quick way to isolate and amplify a specific section of DNA from a mixture. The efforts of the Human Genome Project, Department of Energy, NIH, and parallel private efforts by Celera Genomics led to the sequencing of the human genome in 2003.
Features of inheritanceEdit
Discrete inheritance and Mendel's lawsEdit
- Main article: Mendelian inheritance
At its most fundamental level, inheritance in organisms occurs by passing discrete heritable units, called genes, from parents to offspring. This property was first observed by Gregor Mendel, who studied the segregation of heritable traits in pea plants. In his experiments studying the trait for flower color, Mendel observed that the flowers of each pea plant were either purple or white—but never an intermediate between the two colors. These different, discrete versions of the same gene are called alleles.
In the case of the pea, which is a diploid species, each individual plant has two copies of each gene, one copy inherited from each parent. Many species, including humans, have this pattern of inheritance. Diploid organisms with two copies of the same allele of a given gene are called homozygous at that gene locus, while organisms with two different alleles of a given gene are called heterozygous.
The set of alleles for a given organism is called its genotype, while the observable traits of the organism are called its phenotype. When organisms are heterozygous at a gene, often one allele is called dominant as its qualities dominate the phenotype of the organism, while the other allele is called recessive as its qualities recede and are not observed. Some alleles do not have complete dominance and instead have incomplete dominance by expressing an intermediate phenotype, or codominance by expressing both alleles at once.
When a pair of organisms reproduce sexually, their offspring randomly inherit one of the two alleles from each parent. These observations of discrete inheritance and the segregation of alleles are collectively known as Mendel's first law or the Law of Segregation.
Notation and diagramsEdit
In fertilization and breeding experiments (and especially when discussing Mendel's laws) the parents are referred to as the "P" generation and the offspring as the "F1" (first filial) generation. When the F1 offspring mate with each other, the offspring are called the "F2" (second filial) generation. One of the common diagrams used to predict the result of cross-breeding is the Punnett square.
Multiple gene interactionsEdit
Organisms have thousands of genes, and in sexually reproducing organisms these genes generally assort independently of each other. This means that the inheritance of an allele for yellow or green pea color is unrelated to the inheritance of alleles for white or purple flowers. This phenomenon, known as "Mendel's second law" or the "law of independent assortment," means that the alleles of different genes get shuffled between parents to form offspring with many different combinations. (Some genes do not assort independently, demonstrating genetic linkage, a topic discussed later in this article.)
Often different genes can interact in a way that influences the same trait. In the Blue-eyed Mary (Omphalodes verna), for example, there exists a gene with alleles that determine the color of flowers: blue or magenta. Another gene, however, controls whether the flowers have color at all or are white. When a plant has two copies of this white allele, its flowers are white—regardless of whether the first gene has blue or magenta alleles. This interaction between genes is called epistasis, with the second gene epistatic to the first.
Many traits are not discrete features (e.g. purple or white flowers) but are instead continuous features (e.g. human height and skin color). These complex traits are products of many genes. The influence of these genes is mediated, to varying degrees, by the environment an organism has experienced. The degree to which an organism's genes contribute to a complex trait is called heritability. Measurement of the heritability of a trait is relative—in a more variable environment, the environment has a bigger influence on the total variation of the trait. For example, human height is a trait with complex causes. It has a heritability of 89% in the United States. In Nigeria, however, where people experience a more variable access to good nutrition and health care, height has a heritability of only 62%.
Molecular basis for inheritanceEdit
DNA and chromosomesEdit
- Main article: DNA
The molecular basis for genes is deoxyribonucleic acid (DNA). DNA is composed of a chain of nucleotides, of which there are four types: adenine (A), cytosine (C), guanine (G), and thymine (T). Genetic information exists in the sequence of these nucleotides, and genes exist as stretches of sequence along the DNA chain. Viruses are the only exception to this rule—sometimes viruses use the very similar molecule RNA instead of DNA as their genetic material. Viruses cannot reproduce without a host and are unaffected by many genetic processes, so tend not to be considered living organisms.
DNA normally exists as a double-stranded molecule, coiled into the shape of a double helix. Each nucleotide in DNA preferentially pairs with its partner nucleotide on the opposite strand: A pairs with T, and C pairs with G. Thus, in its two-stranded form, each strand effectively contains all necessary information, redundant with its partner strand. This structure of DNA is the physical basis for inheritance: DNA replication duplicates the genetic information by splitting the strands and using each strand as a template for synthesis of a new partner strand.
Genes are arranged linearly along long chains of DNA base-pair sequences. In bacteria, each cell usually contains a single circular genophore, while eukaryotic organisms (such as plants and animals) have their DNA arranged in multiple linear chromosomes. These DNA strands are often extremely long; the largest human chromosome, for example, is about 247 million base pairs in length. The DNA of a chromosome is associated with structural proteins that organize, compact, and control access to the DNA, forming a material called chromatin; in eukaryotes, chromatin is usually composed of nucleosomes, segments of DNA wound around cores of histone proteins. The full set of hereditary material in an organism (usually the combined DNA sequences of all chromosomes) is called the genome.
While haploid organisms have only one copy of each chromosome, most animals and many plants are diploid, containing two of each chromosome and thus two copies of every gene. The two alleles for a gene are located on identical loci of the two homologous chromosomes, each allele inherited from a different parent.
Many species have so-called sex chromosomes that determine the gender of each organism. In humans and many other animals, the Y chromosome contains the gene that triggers the development of the specifically male characteristics. In evolution, this chromosome has lost most of its content and also most of its genes, while the X chromosome is similar to the other chromosomes and contains many genes. The X and Y chromosomes form a strongly heterogeneous pair.
- Main article: Asexual reproduction
When cells divide, their full genome is copied and each daughter cell inherits one copy. This process, called mitosis, is the simplest form of reproduction and is the basis for asexual reproduction. Asexual reproduction can also occur in multicellular organisms, producing offspring that inherit their genome from a single parent. Offspring that are genetically identical to their parents are called clones.
Eukaryotic organisms often use sexual reproduction to generate offspring that contain a mixture of genetic material inherited from two different parents. The process of sexual reproduction alternates between forms that contain single copies of the genome (haploid) and double copies (diploid). Haploid cells fuse and combine genetic material to create a diploid cell with paired chromosomes. Diploid organisms form haploids by dividing, without replicating their DNA, to create daughter cells that randomly inherit one of each pair of chromosomes. Most animals and many plants are diploid for most of their lifespan, with the haploid form reduced to single cell gametes such as sperm or eggs.
Although they do not use the haploid/diploid method of sexual reproduction, bacteria have many methods of acquiring new genetic information. Some bacteria can undergo conjugation, transferring a small circular piece of DNA to another bacterium. Bacteria can also take up raw DNA fragments found in the environment and integrate them into their genomes, a phenomenon known as transformation. These processes result in horizontal gene transfer, transmitting fragments of genetic information between organisms that would be otherwise unrelated.
- Main article: Chromosomal crossover
The diploid nature of chromosomes allows for genes on different chromosomes to assort independently or be separated from their homologous pair during sexual reproduction wherein haploid gametes are formed. In this way new combinations of genes can occur in the offspring of a mating pair. Genes on the same chromosome would theoretically never recombine. However, they do, via the cellular process of chromosomal crossover. During crossover, chromosomes exchange stretches of DNA, effectively shuffling the gene alleles between the chromosomes. This process of chromosomal crossover generally occurs during meiosis, a series of cell divisions that creates haploid cells.
The first cytological demonstration of crossing over was performed by Harriet Creighton and Barbara McClintock in 1931. Their research and experiments on corn provided cytological evidence for the genetic theory that linked genes on paired chromosomes do in fact exchange places from one homolog to the other.
The probability of chromosomal crossover occurring between two given points on the chromosome is related to the distance between the points. For an arbitrarily long distance, the probability of crossover is high enough that the inheritance of the genes is effectively uncorrelated. For genes that are closer together, however, the lower probability of crossover means that the genes demonstrate genetic linkage; alleles for the two genes tend to be inherited together. The amounts of linkage between a series of genes can be combined to form a linear linkage map that roughly describes the arrangement of the genes along the chromosome.
- Main article: Genetic code
Genes generally express their functional effect through the production of proteins, which are complex molecules responsible for most functions in the cell. Proteins are made up of one or more polypeptide chains, each of which is composed of a sequence of amino acids, and the DNA sequence of a gene (through an RNA intermediate) is used to produce a specific amino acid sequence. This process begins with the production of an RNA molecule with a sequence matching the gene's DNA sequence, a process called transcription.
This messenger RNA molecule is then used to produce a corresponding amino acid sequence through a process called translation. Each group of three nucleotides in the sequence, called a codon, corresponds either to one of the twenty possible amino acids in a protein or an instruction to end the amino acid sequence; this correspondence is called the genetic code. The flow of information is unidirectional: information is transferred from nucleotide sequences into the amino acid sequence of proteins, but it never transfers from protein back into the sequence of DNA—a phenomenon Francis Crick called the central dogma of molecular biology.
The specific sequence of amino acids results in a unique three-dimensional structure for that protein, and the three-dimensional structures of proteins are related to their functions. Some are simple structural molecules, like the fibers formed by the protein collagen. Proteins can bind to other proteins and simple molecules, sometimes acting as enzymes by facilitating chemical reactions within the bound molecules (without changing the structure of the protein itself). Protein structure is dynamic; the protein hemoglobin bends into slightly different forms as it facilitates the capture, transport, and release of oxygen molecules within mammalian blood.
A single nucleotide difference within DNA can cause a change in the amino acid sequence of a protein. Because protein structures are the result of their amino acid sequences, some changes can dramatically change the properties of a protein by destabilizing the structure or changing the surface of the protein in a way that changes its interaction with other proteins and molecules. For example, sickle-cell anemia is a human genetic disease that results from a single base difference within the coding region for the β-globin section of hemoglobin, causing a single amino acid change that changes hemoglobin's physical properties. Sickle-cell versions of hemoglobin stick to themselves, stacking to form fibers that distort the shape of red blood cells carrying the protein. These sickle-shaped cells no longer flow smoothly through blood vessels, having a tendency to clog or degrade, causing the medical problems associated with this disease.
Some DNA sequences are transcribed into RNA but are not translated into protein products—such RNA molecules are called non-coding RNA. In some cases, these products fold into structures which are involved in critical cell functions (e.g. ribosomal RNA and transfer RNA). RNA can also have regulatory effects through hybridization interactions with other RNA molecules (e.g. microRNA).
Nature and nurtureEdit
- Main article: Nature and nurture
Although genes contain all the information an organism uses to function, the environment plays an important role in determining the ultimate phenotypes an organism displays. The phrase "nature and nurture" refers to this complementary relationship. The phenotype of an organism depends on the interaction of genes and the environment. An interesting example is the coat coloration of the Siamese cat. In this case, the body temperature of the cat plays the role of the environment. The cat's genes code for dark hair, thus the hair-producing cells in the cat make cellular proteins resulting in dark hair. But these dark hair-producing proteins are sensitive to temperature (i.e. have a mutation causing temperature-sensitivity) and denature in higher-temperature environments, failing to produce dark-hair pigment in areas where the cat has a higher body temperature. In a low-temperature environment, however, the protein's structure is stable and produces dark-hair pigment normally. The protein remains functional in areas of skin that are colder—such as its legs, ears, tail and face—so the cat has dark-hair at its extremities.
Environment plays a major role in effects of the human genetic disease phenylketonuria. The mutation that causes phenylketonuria disrupts the ability of the body to break down the amino acid phenylalanine, causing a toxic build-up of an intermediate molecule that, in turn, causes severe symptoms of progressive intellectual disability and seizures. However, if someone with the phenylketonuria mutation follows a strict diet that avoids this amino acid, they remain normal and healthy.
A common method for determining how genes and environment ("nature and nurture") contribute to a phenotype involves studying identical and fraternal twins, or other siblings of multiple births. Because identical siblings come from the same zygote, they are genetically the same. Fraternal twins are as genetically different from one another as normal siblings. By comparing how often a certain disorder occurs in a pair of identical twins to how often it occurs in a pair of fraternal twins, scientists can determine whether that disorder is caused by genetic or postnatal environmental factors – whether it has "nature" or "nurture" causes. One famous example involved the study of the Genain quadruplets, who were identical quadruplets all diagnosed with schizophrenia. However such tests cannot separate genetic factors from environmental factors affecting fetal development.
- Main article: Regulation of gene expression
The genome of a given organism contains thousands of genes, but not all these genes need to be active at any given moment. A gene is expressed when it is being transcribed into mRNA and there exist many cellular methods of controlling the expression of genes such that proteins are produced only when needed by the cell. Transcription factors are regulatory proteins that bind to DNA, either promoting or inhibiting the transcription of a gene. Within the genome of Escherichia coli bacteria, for example, there exists a series of genes necessary for the synthesis of the amino acid tryptophan. However, when tryptophan is already available to the cell, these genes for tryptophan synthesis are no longer needed. The presence of tryptophan directly affects the activity of the genes—tryptophan molecules bind to the tryptophan repressor (a transcription factor), changing the repressor's structure such that the repressor binds to the genes. The tryptophan repressor blocks the transcription and expression of the genes, thereby creating negative feedback regulation of the tryptophan synthesis process.
Differences in gene expression are especially clear within multicellular organisms, where cells all contain the same genome but have very different structures and behaviors due to the expression of different sets of genes. All the cells in a multicellular organism derive from a single cell, differentiating into variant cell types in response to external and intercellular signals and gradually establishing different patterns of gene expression to create different behaviors. As no single gene is responsible for the development of structures within multicellular organisms, these patterns arise from the complex interactions between many cells.
Within eukaryotes, there exist structural features of chromatin that influence the transcription of genes, often in the form of modifications to DNA and chromatin that are stably inherited by daughter cells. These features are called "epigenetic" because they exist "on top" of the DNA sequence and retain inheritance from one cell generation to the next. Because of epigenetic features, different cell types grown within the same medium can retain very different properties. Although epigenetic features are generally dynamic over the course of development, some, like the phenomenon of paramutation, have multigenerational inheritance and exist as rare exceptions to the general rule of DNA as the basis for inheritance.
- Main article: Mutation
During the process of DNA replication, errors occasionally occur in the polymerization of the second strand. These errors, called mutations, can affect the phenotype of an organism, especially if they occur within the protein coding sequence of a gene. Error rates are usually very low—1 error in every 10–100 million bases—due to the "proofreading" ability of DNA polymerases. Processes that increase the rate of changes in DNA are called mutagenic: mutagenic chemicals promote errors in DNA replication, often by interfering with the structure of base-pairing, while UV radiation induces mutations by causing damage to the DNA structure. Chemical damage to DNA occurs naturally as well and cells use DNA repair mechanisms to repair mismatches and breaks. The repair does not, however, always restore the original sequence.
In organisms that use chromosomal crossover to exchange DNA and recombine genes, errors in alignment during meiosis can also cause mutations. Errors in crossover are especially likely when similar sequences cause partner chromosomes to adopt a mistaken alignment; this makes some regions in genomes more prone to mutating in this way. These errors create large structural changes in DNA sequence – duplications, inversions, deletions of entire regions – or the accidental exchange of whole parts of sequences between different chromosomes (chromosomal translocation).
Natural selection and evolutionEdit
- Main article: Evolution
Mutations alter an organism's genotype and occasionally this causes different phenotypes to appear. Most mutations have little effect on an organism's phenotype, health, or reproductive fitness. Mutations that do have an effect are usually detrimental, but occasionally some can be beneficial. Studies in the fly Drosophila melanogaster suggest that if a mutation changes a protein produced by a gene, about 70 percent of these mutations will be harmful with the remainder being either neutral or weakly beneficial.
Population genetics studies the distribution of genetic differences within populations and how these distributions change over time. Changes in the frequency of an allele in a population are mainly influenced by natural selection, where a given allele provides a selective or reproductive advantage to the organism, as well as other factors such as mutation, genetic drift, genetic draft, artificial selection and migration.
Over many generations, the genomes of organisms can change significantly, resulting in evolution. In the process called adaptation, selection for beneficial mutations can cause a species to evolve into forms better able to survive in their environment. New species are formed through the process of speciation, often caused by geographical separations that prevent populations from exchanging genes with each other.
By comparing the homology between different species' genomes, it is possible to calculate the evolutionary distance between them and when they may have diverged. Genetic comparisons are generally considered a more accurate method of characterizing the relatedness between species than the comparison of phenotypic characteristics. The evolutionary distances between species can be used to form evolutionary trees; these trees represent the common descent and divergence of species over time, although they do not show the transfer of genetic material between unrelated species (known as horizontal gene transfer and most common in bacteria).
Although geneticists originally studied inheritance in a wide range of organisms, researchers began to specialize in studying the genetics of a particular subset of organisms. The fact that significant research already existed for a given organism would encourage new researchers to choose it for further study, and so eventually a few model organisms became the basis for most genetics research. Common research topics in model organism genetics include the study of gene regulation and the involvement of genes in development and cancer.
Organisms were chosen, in part, for convenience—short generation times and easy genetic manipulation made some organisms popular genetics research tools. Widely used model organisms include the gut bacterium Escherichia coli, the plant Arabidopsis thaliana, baker's yeast (Saccharomyces cerevisiae), the nematode Caenorhabditis elegans, the common fruit fly (Drosophila melanogaster), and the common house mouse (Mus musculus).
Medical genetics seeks to understand how genetic variation relates to human health and disease. When searching for an unknown gene that may be involved in a disease, researchers commonly use genetic linkage and genetic pedigree charts to find the location on the genome associated with the disease. At the population level, researchers take advantage of Mendelian randomization to look for locations in the genome that are associated with diseases, a method especially useful for multigenic traits not clearly defined by a single gene. Once a candidate gene is found, further research is often done on the corresponding (or homologous) genes of model organisms. In addition to studying genetic diseases, the increased availability of genotyping methods has led to the field of pharmacogenetics: the study of how genotype can affect drug responses.
Individuals differ in their inherited tendency to develop cancer, and cancer is a genetic disease. The process of cancer development in the body is a combination of events. Mutations occasionally occur within cells in the body as they divide. Although these mutations will not be inherited by any offspring, they can affect the behavior of cells, sometimes causing them to grow and divide more frequently. There are biological mechanisms that attempt to stop this process; signals are given to inappropriately dividing cells that should trigger cell death, but sometimes additional mutations occur that cause cells to ignore these messages. An internal process of natural selection occurs within the body and eventually mutations accumulate within cells to promote their own growth, creating a cancerous tumor that grows and invades various tissues of the body.
Normally, a cell divides only in response to signals called growth factors and stops growing once in contact with surrounding cells and in response to growth-inhibitory signals. It usually then divides a limited number of times and dies, staying within the epithelium where it is unable to migrate to other organs. To become a cancer cell, a cell has to accumulate mutations in a number of genes (three to seven) that allow it to bypass this regulation: it no longer needs growth factors to divide, continues growing when making contact to neighbor cells, ignores inhibitory signals, keeps growing indefinitely and is immortal, escapes from the epithelium and ultimately may be able to escape from the primary tumor, cross the endothelium of a blood vessel, be transported by the bloodstream and colonize a new organ, forming deadly metastasis. Although there are some genetic predispositions in a small fraction of cancers, the major fraction is due to a set of new genetic mutations that originally appear and accumulate in one or a small number of cells that will divide to form the tumor and are not transmitted to the progeny (somatic mutations). The most frequent mutations are a loss of function of p53 protein, a tumor suppressor, or in the p53 pathway, and gain of function mutations in the Ras proteins, or in other oncogenes.
DNA can be manipulated in the laboratory. Restriction enzymes are commonly used enzymes that cut DNA at specific sequences, producing predictable fragments of DNA. DNA fragments can be visualized through use of gel electrophoresis, which separates fragments according to their length.
The use of ligation enzymes allows DNA fragments to be connected. By binding ("ligating") fragments of DNA together from different sources, researchers can create recombinant DNA, the DNA often associated with genetically modified organisms. Recombinant DNA is commonly used in the context of plasmids: short circular DNA molecules with a few genes on them. In the process known as molecular cloning, researchers can amplify the DNA fragments by inserting plasmids into bacteria and then culturing them on plates of agar (to isolate clones of bacteria cells – "cloning" can also refer to the various means of creating cloned ("clonal") organisms).
DNA can also be amplified using a procedure called the polymerase chain reaction (PCR). By using specific short sequences of DNA, PCR can isolate and exponentially amplify a targeted region of DNA. Because it can amplify from extremely small amounts of DNA, PCR is also often used to detect the presence of specific DNA sequences.
DNA sequencing and genomicsEdit
DNA sequencing, one of the most fundamental technologies developed to study genetics, allows researchers to determine the sequence of nucleotides in DNA fragments. The technique of chain-termination sequencing, developed in 1977 by a team led by Frederick Sanger, is still routinely used to sequence DNA fragments. Using this technology, researchers have been able to study the molecular sequences associated with many human diseases.
As sequencing has become less expensive, researchers have sequenced the genomes of many organisms using a process called genome assembly, which utilizes computational tools to stitch together sequences from many different fragments. These technologies were used to sequence the human genome in the Human Genome Project completed in 2003. New high-throughput sequencing technologies are dramatically lowering the cost of DNA sequencing, with many researchers hoping to bring the cost of resequencing a human genome down to a thousand dollars.
Next-generation sequencing (or high-throughput sequencing) came about due to the ever-increasing demand for low-cost sequencing. These sequencing technologies allow the production of potentially millions of sequences concurrently. The large amount of sequence data available has created the field of genomics, research that uses computational tools to search for and analyze patterns in the full genomes of organisms. Genomics can also be considered a subfield of bioinformatics, which uses computational approaches to analyze large sets of biological data. A common problem to these fields of research is how to manage and share data that deals with human subject and personally identifiable information. See also genomics data sharing.
Society and cultureEdit
On 19 March 2015, a group of leading biologists urged a worldwide ban on clinical use of methods, particularly the use of CRISPR and zinc finger, to edit the human genome in a way that can be inherited. In April 2015, Chinese researchers reported results of basic research to edit the DNA of non-viable human embryos using CRISPR.
- Bacterial genome size
- Cryoconservation of animal genetic resources
- Genetic disorder
- Genetic diversity
- Genetic engineering
- Genetic enhancement
- Index of genetics articles
- Medical genetics
- Molecular tools for gene study
- Outline of genetics
- Timeline of the history of genetics
- ↑ Griffiths, Anthony J. F.; Miller, Jeffrey H.; Suzuki, David T. et al., eds. (2000). "Genetics and the Organism: Introduction". An Introduction to Genetic Analysis (7th ed.). New York: W. H. Freeman. ISBN 0-7167-3520-2. https://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=iga.section.60.
- ↑ Hartl D, Jones E (2005)
- ↑ "Genetikos (γενετ-ικός)". Henry George Liddell, Robert Scott, A Greek-English Lexicon. Perseus Digital Library, Tufts University. http://www.perseus.tufts.edu/hopper/text?doc=Perseus%3Atext%3A1999.04.0057%3Aentry%3D%2321880&redirect=true. Retrieved 20 February 2012.
- ↑ "Genesis (γένεσις)". Henry George Liddell, Robert Scott, A Greek-English Lexicon. Perseus Digital Library, Tufts University. http://www.perseus.tufts.edu/hopper/text?doc=Perseus%3Atext%3A1999.04.0057%3Aentry%3D%2321873&redirect=true. Retrieved 20 February 2012.
- ↑ "Genetic". Online Etymology Dictionary. http://www.etymonline.com/index.php?search=Genetic&searchmode=none. Retrieved 20 February 2012.
- ↑ DK Publishing (2009). Science: The Definitive Visual Guide. Penguin. p. 362. ISBN 978-0-7566-6490-9. https://books.google.com/books?id=sFiJFuzRVFQC&pg=PA362.
- ↑ Weiling, F (1991). "Historical study: Johann Gregor Mendel 1822–1884.". American Journal of Medical Genetics 40 (1): 1–25; discussion 26. doi:10.1002/ajmg.1320400103. PMID 1887835.
- ↑ Poczai P.; Bell N.; Hyvönen J. (2014). "Imre Festetics and the Sheep Breeders' Society of Moravia: Mendel's Forgotten "Research Network"". PLoS Biology 12 (1): e1001772. doi:10.1371/journal.pbio.1001772. PMC 3897355. PMID 24465180. http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1001772.
- ↑ Matthew Hamilton (2011). Population Genetics. Georgetown University. p. 26. ISBN 978-1-4443-6245-9. https://books.google.com/books?id=ng85sd1UR7EC&pg=PT26.
- ↑ Lamarck, J-B (2008). In Encyclopædia Britannica. Retrieved from Encyclopædia Britannica Online on 16 March 2008.
- ↑ Singer, Emily (4 February 2009). "A Comeback for Lamarckian Evolution?". Technology Review. http://www.technologyreview.com/news/411880/a-comeback-for-lamarckian-evolution/. Retrieved 14 March 2013.
- ↑ Peter J. Bowler, The Mendelian Revolution: The Emergency of Hereditarian Concepts in Modern Science and Society (Baltimore: Johns Hopkins University Press, 1989): chapters 2 & 3.
- ↑ 13.0 13.1 Blumberg, Roger B.. "Mendel's Paper in English". http://www.mendelweb.org/Mendel.html.
- ↑ genetics, n., Oxford English Dictionary, 3rd ed.
- ↑ Bateson W. "Letter from William Bateson to Alan Sedgwick in 1905". The John Innes Centre. Archived from the original on 13 October 2007. https://web.archive.org/web/20071013020831/http://www.jic.ac.uk/corporate/about/bateson.htm. Retrieved 15 March 2008. Note that the letter was to an Adam Sedgwick, a zoologist and "Reader in Animal Morphology" at Trinity College, Cambridge
- ↑ genetic, adj., Oxford English Dictionary, 3rd ed.
- ↑ Richmond, Marsha L (November 2007). "Opportunities for women in early genetics". Nature Reviews Genetics 8 (11): 897–902. doi:10.1038/nrg2200. PMID 17893692. http://www.nature.com/reviews/genetics.
- ↑ Script error :Initially titled the "International Conference on Hybridisation and Plant Breeding", the title was changed as a result of Bateson's speech. See: Treasure your exceptions: the science and life of William Bateson. Springer. 2008. p. 248. ISBN 978-0-387-75687-5.
- ↑ Moore, John A. (1983). "Thomas Hunt Morgan—The Geneticist". Integrative and Comparative Biology 23 (4): 855–865. doi:10.1093/icb/23.4.855.
- ↑ Sturtevant AH (1913). "The linear arrangement of six sex-linked factors in Drosophila, as shown by their mode of association". Journal of Experimental Biology 14: 43–59. doi:10.1002/jez.1400140104. http://www.esp.org/foundations/genetics/classical/holdings/s/ahs-13.pdf.
- ↑ Avery, OT; MacLeod, CM; McCarty, M (1944). "Studies on the Chemical Nature of the Substance Inducing Transformation of Pneumococcal Types: Induction of Transformation by a Desoxyribonucleic Acid Fraction Isolated from Pneumococcus Type III". The Journal of Experimental Medicine 79 (2): 137–58. doi:10.1084/jem.79.2.137. PMC 2135445. PMID 19871359. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2135445. Reprint: Avery, OT; MacLeod, CM; McCarty, M (1979). "Studies on the chemical nature of the substance inducing transformation of pneumococcal types. Inductions of transformation by a desoxyribonucleic acid fraction isolated from pneumococcus type III". The Journal of Experimental Medicine 149 (2): 297–326. doi:10.1084/jem.149.2.297. PMC 2184805. PMID 33226. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2184805.
- ↑ "Cell and Molecular Biology", Pragya Khanna. I. K. International Pvt Ltd, 2008. p. 221. ISBN 81-89866-59-1Script error, ISBN 978-81-89866-59-4Script error
- ↑ Hershey, AD; Chase, M (1952). "Independent functions of viral protein and nucleic acid in growth of bacteriophage". The Journal of General Physiology 36 (1): 39–56. doi:10.1085/jgp.36.1.39. PMC 2147348. PMID 12981234. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2147348.
- ↑ Judson, Horace (1979). The Eighth Day of Creation: Makers of the Revolution in Biology. Cold Spring Harbor Laboratory Press. pp. 51–169. ISBN 0-87969-477-7.
- ↑ Watson, J. D.; Crick, FH (1953). "Molecular Structure of Nucleic Acids: A Structure for Deoxyribose Nucleic Acid". Nature 171 (4356): 737–8. Bibcode 1953Natur.171..737W. doi:10.1038/171737a0. PMID 13054692. http://www.nature.com/nature/dna50/watsoncrick.pdf.
- ↑ Watson, J. D.; Crick, FH (1953). "Genetical Implications of the Structure of Deoxyribonucleic Acid". Nature 171 (4361): 964–7. Bibcode 1953Natur.171..964W. doi:10.1038/171964b0. PMID 13063483. http://www.nature.com/nature/dna50/watsoncrick2.pdf.
- ↑ Stratmann, S. A. (1 Nov 2013). "DNA replication at the single molecule level". Chemical Society Reviews 43 (4): 1201–20. doi:10.1039/c3cs60391a. PMID 24395040.
- ↑ Frederick Betz (2010). Managing Science: Methodology and Organization of Research. Springer. p. 76. ISBN 978-1-4419-7488-4. https://books.google.com/books?id=1ARRexcXgAgC&pg=PA76.
- ↑ Stanley A. Rice (2009). Encyclopedia of Evolution. Infobase Publishing. p. 134. ISBN 978-1-4381-1005-9. https://books.google.com/books?id=YRcAVvmE6eMC&pg=PA134.
- ↑ Sahotra Sarkar (1998). Genetics and Reductionism. Cambridge University Press. p. 140. ISBN 978-0-521-63713-8. https://books.google.com/books?id=7lzpDHFw-40C&pg=PA140.
- ↑ Ohta, Tomoko (1973). "Slightly Deleterious Mutant Substitutions in Evolution". Nature 246 (5428): 96–98. Bibcode 1973Natur.246...96O. doi:10.1038/246096a0. PMID 4585855.
- ↑ Sanger, F; Nicklen, S; Coulson, AR (1977). "DNA sequencing with chain-terminating inhibitors". Proceedings of the National Academy of Sciences of the United States of America 74 (12): 5463–7. Bibcode 1977PNAS...74.5463S. doi:10.1073/pnas.74.12.5463. PMC 431765. PMID 271968. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=431765.
- ↑ Saiki, RK; Scharf, S; Faloona, F; Mullis, KB; Horn, GT; Erlich, HA; Arnheim, N (1985). "Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia". Science 230 (4732): 1350–4. Bibcode 1985Sci...230.1350S. doi:10.1126/science.2999980. PMID 2999980.
- ↑ 34.0 34.1 "Human Genome Project Information". Human Genome Project. http://www.ornl.gov/sci/techresources/Human_Genome/home.shtml. Retrieved 15 March 2008.
- ↑ "The sequence of the human genome". Science 291.
- ↑ Griffiths, Anthony J. F.; Miller, Jeffrey H.; Suzuki, David T. et al., eds. (2000). "Patterns of Inheritance: Introduction". An Introduction to Genetic Analysis (7th ed.). New York: W. H. Freeman. ISBN 0-7167-3520-2. https://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=iga.section.199.
- ↑ Griffiths, Anthony J. F.; Miller, Jeffrey H.; Suzuki, David T. et al., eds. (2000). "Mendel's experiments". An Introduction to Genetic Analysis (7th ed.). New York: W. H. Freeman. ISBN 0-7167-3520-2. https://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=iga.section.200.
- ↑ 38.0 38.1 38.2 Griffiths, Anthony J. F.; Miller, Jeffrey H.; Suzuki, David T. et al., eds. (2000). "Mendelian genetics in eukaryotic life cycles". An Introduction to Genetic Analysis (7th ed.). New York: W. H. Freeman. ISBN 0-7167-3520-2. https://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=iga.section.484.
- ↑ Griffiths, Anthony J. F.; Miller, Jeffrey H.; Suzuki, David T. et al., eds. (2000). "Interactions between the alleles of one gene". An Introduction to Genetic Analysis (7th ed.). New York: W. H. Freeman. ISBN 0-7167-3520-2. https://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=iga.section.630.
- ↑ Cheney, Richard W.. "Genetic Notation". Christopher Newport University. Archived from the original on 3 January 2008. https://web.archive.org/web/20080103021518/http://faculty.users.cnu.edu/rcheney/Genetic%20Notation.htm. Retrieved 18 March 2008.
- ↑ Griffiths, Anthony J. F.; Miller, Jeffrey H.; Suzuki, David T. et al., eds. (2000). "Human Genetics". An Introduction to Genetic Analysis (7th ed.). New York: W. H. Freeman. ISBN 0-7167-3520-2. https://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=iga.section.229.
- ↑ Griffiths, Anthony J. F.; Miller, Jeffrey H.; Suzuki, David T. et al., eds. (2000). "Gene interaction and modified dihybrid ratios". An Introduction to Genetic Analysis (7th ed.). New York: W. H. Freeman. ISBN 0-7167-3520-2. https://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=iga.section.644.
- ↑ Mayeux, R (2005). "Mapping the new frontier: complex genetic disorders". The Journal of Clinical Investigation 115 (6): 1404–7. doi:10.1172/JCI25421. PMC 1137013. PMID 15931374. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1137013.
- ↑ Griffiths, Anthony J. F.; Miller, Jeffrey H.; Suzuki, David T. et al., eds. (2000). "Quantifying heritability". An Introduction to Genetic Analysis (7th ed.). New York: W. H. Freeman. ISBN 0-7167-3520-2. https://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=iga.section.4009.
- ↑ Luke, A; Guo, X; Adeyemo, AA; Wilks, R; Forrester, T; Lowe Jr, W; Comuzzie, AG; Martin, LJ et al. (2001). "Heritability of obesity-related traits among Nigerians, Jamaicans and US black people". International Journal of Obesity and Related Metabolic Disorders 25 (7): 1034–41. doi:10.1038/sj.ijo.0801650. PMID 11443503.
- ↑ Pearson, H (2006). "Genetics: what is a gene?". Nature 441 (7092): 398–401. Bibcode 2006Natur.441..398P. doi:10.1038/441398a. PMID 16724031.
- ↑ Prescott, L (1993). Microbiology. Wm. C. Brown Publishers. ISBN 0-697-01372-3.
- ↑ Griffiths, Anthony J. F.; Miller, Jeffrey H.; Suzuki, David T. et al., eds. (2000). "Mechanism of DNA Replication". An Introduction to Genetic Analysis (7th ed.). New York: W. H. Freeman. ISBN 0-7167-3520-2. https://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=iga.section.1523.
- ↑ Gregory, SG; Barlow, KF; McLay, KE; Kaul, R; Swarbreck, D; Dunham, A; Scott, CE; Howe, KL et al. (2006). "The DNA sequence and biological annotation of human chromosome 1". Nature 441 (7091): 315–21. Bibcode 2006Natur.441..315G. doi:10.1038/nature04727. PMID 16710414.
- ↑ Alberts et al. (2002), II.4. DNA and chromosomes: Chromosomal DNA and Its Packaging in the Chromatin Fiber
- ↑ Griffiths, Anthony J. F.; Miller, Jeffrey H.; Suzuki, David T. et al., eds. (2000). "Sex chromosomes and sex-linked inheritance". An Introduction to Genetic Analysis (7th ed.). New York: W. H. Freeman. ISBN 0-7167-3520-2. https://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=iga.section.222.
- ↑ Griffiths, Anthony J. F.; Miller, Jeffrey H.; Suzuki, David T. et al., eds. (2000). "Bacterial conjugation". An Introduction to Genetic Analysis (7th ed.). New York: W. H. Freeman. ISBN 0-7167-3520-2. https://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=iga.section.1304.
- ↑ Griffiths, Anthony J. F.; Miller, Jeffrey H.; Suzuki, David T. et al., eds. (2000). "Bacterial transformation". An Introduction to Genetic Analysis (7th ed.). New York: W. H. Freeman. ISBN 0-7167-3520-2. https://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=iga.section.1343.
- ↑ Griffiths, Anthony J. F.; Miller, Jeffrey H.; Suzuki, David T. et al., eds. (2000). "Nature of crossing-over". An Introduction to Genetic Analysis (7th ed.). New York: W. H. Freeman. ISBN 0-7167-3520-2. https://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=iga.section.929.
- ↑ "A Correlation of Cytological and Genetical Crossing-Over in Zea Mays". Proc Natl Acad Sci USA 17.
- ↑ Jack E. Staub (1994). Crossover: Concepts and Applications in Genetics, Evolution, and Breeding. University of Wisconsin Press. p. 55. ISBN 978-0-299-13564-5. https://books.google.com/books?id=R43qWg5A-GsC&pg=PA55.
- ↑ Griffiths, Anthony J. F.; Miller, Jeffrey H.; Suzuki, David T. et al., eds. (2000). "Linkage maps". An Introduction to Genetic Analysis (7th ed.). New York: W. H. Freeman. ISBN 0-7167-3520-2. https://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=iga.section.899.
- ↑ "I. 5. DNA, RNA, and the Flow of Genetic Information: Amino Acids Are Encoded by Groups of Three Bases Starting from a Fixed Point". Biochemistry (5th ed.). New York: W. H. Freeman and Company. 2002. https://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=stryer.section.685.
- ↑ Crick, F (1970). "Central dogma of molecular biology". Nature 227 (5258): 561–3. Bibcode 1970Natur.227..561C. doi:10.1038/227561a0. PMID 4913914. http://www.nature.com/nature/focus/crick/pdf/crick227.pdf.
- ↑ Alberts et al. (2002), I.3. Proteins: The Shape and Structure of Proteins
- ↑ Alberts et al. (2002), I.3. Proteins: Protein Function
- ↑ "How Does Sickle Cell Cause Disease?". Brigham and Women's Hospital: Information Center for Sickle Cell and Thalassemic Disorders. 11 April 2002. http://sickle.bwh.harvard.edu/scd_background.html. Retrieved 23 July 2007.
- ↑ Imes, DL; Geary, LA; Grahn, RA; Lyons, LA (2006). "Albinism in the domestic cat (Felis catus) is associated with a tyrosinase (TYR) mutation". Animal Genetics 37 (2): 175–8. doi:10.1111/j.1365-2052.2005.01409.x. PMC 1464423. PMID 16573534. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1464423.
- ↑ "MedlinePlus: Phenylketonuria". NIH: National Library of Medicine. https://www.nlm.nih.gov/medlineplus/phenylketonuria.html. Retrieved 15 March 2008.
- ↑ For example, Ridley, M. (2003). Nature via Nurture: Genes, Experience and What Makes Us Human. Fourth Estate. p. 73. ISBN 978-1-84115-745-0.
- ↑ Rosenthal, David (1964). The Genain Quadruplets: A Case Study and Theoretical Analysis of Heredity and Environment in Schizophrenia. New York: Basic Books. doi:10.1002/bs.3830090407.
- ↑ Brivanlou, AH; Darnell Jr, JE (2002). "Signal transduction and the control of gene expression". Science 295 (5556): 813–8. Bibcode 2002Sci...295..813B. doi:10.1126/science.1066355. PMID 11823631.
- ↑ Alberts et al. (2002), II.3. Control of Gene Expression – The Tryptophan Repressor is a Simple Switch That Turns Genes On and Off in Bacteria
- ↑ Jaenisch, R; Bird, A (2003). "Epigenetic regulation of gene expression: how the genome integrates intrinsic and environmental signals". Nature Genetics 33 Suppl (3s): 245–54. doi:10.1038/ng1089. PMID 12610534.
- ↑ Chandler, VL (2007). "Paramutation: from maize to mice". Cell 128 (4): 641–5. doi:10.1016/j.cell.2007.02.007. PMID 17320501.
- ↑ Griffiths, Anthony J. F.; Miller, Jeffrey H.; Suzuki, David T. et al., eds. (2000). "Spontaneous mutations". An Introduction to Genetic Analysis (7th ed.). New York: W. H. Freeman. ISBN 0-7167-3520-2. https://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=iga.section.2706.
- ↑ Freisinger, E; Grollman, AP; Miller, H; Kisker, C (2004). "Lesion (in)tolerance reveals insights into DNA replication fidelity". The EMBO Journal 23 (7): 1494–505. doi:10.1038/sj.emboj.7600158. PMC 391067. PMID 15057282. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=391067.
- ↑ Griffiths, Anthony J. F.; Miller, Jeffrey H.; Suzuki, David T. et al., eds. (2000). "Induced mutations". An Introduction to Genetic Analysis (7th ed.). New York: W. H. Freeman. ISBN 0-7167-3520-2. https://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=iga.section.2727.
- ↑ Griffiths, Anthony J. F.; Miller, Jeffrey H.; Suzuki, David T. et al., eds. (2000). "Chromosome Mutation I: Changes in Chromosome Structure: Introduction". An Introduction to Genetic Analysis (7th ed.). New York: W. H. Freeman. ISBN 0-7167-3520-2. https://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=iga.section.2844.
- ↑ Moselio Schaechter (2009). Encyclopedia of Microbiology. Academic Press. p. 551. ISBN 978-0-12-373944-5. https://books.google.com/books?id=rLhdW5YzuO4C&pg=RA1-PA551.
- ↑ Mike Calver; Alan Lymbery; Jennifer McComb; Mike Bamford (2009). Environmental Biology. Cambridge University Press. p. 118. ISBN 978-0-521-67982-4. https://books.google.com/books?id=HemnRxzdiFQC&pg=PA118.
- ↑ Sawyer, SA; Parsch, J; Zhang, Z; Hartl, DL (2007). "Prevalence of positive selection among nearly neutral amino acid replacements in Drosophila". Proceedings of the National Academy of Sciences of the United States of America 104 (16): 6504–10. Bibcode 2007PNAS..104.6504S. doi:10.1073/pnas.0701572104. PMC 1871816. PMID 17409186. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1871816.
- ↑ Griffiths, Anthony J. F.; Miller, Jeffrey H.; Suzuki, David T. et al., eds. (2000). "Variation and its modulation". An Introduction to Genetic Analysis (7th ed.). New York: W. H. Freeman. ISBN 0-7167-3520-2. https://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=iga.section.3842.
- ↑ Griffiths, Anthony J. F.; Miller, Jeffrey H.; Suzuki, David T. et al., eds. (2000). "Selection". An Introduction to Genetic Analysis (7th ed.). New York: W. H. Freeman. ISBN 0-7167-3520-2. https://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=iga.section.3886.
- ↑ Gillespie, John H. (2001). "Is the population size of a species relevant to its evolution?". Evolution 55 (11): 2161–2169. doi:10.1111/j.0014-3820.2001.tb00732.x. PMID 11794777.
- ↑ Griffiths, Anthony J. F.; Miller, Jeffrey H.; Suzuki, David T. et al., eds. (2000). "Random events". An Introduction to Genetic Analysis (7th ed.). New York: W. H. Freeman. ISBN 0-7167-3520-2. https://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=iga.section.3906.
- ↑ Darwin, Charles (1859). On the Origin of Species (1st ed.). London: John Murray. pp. 1. ISBN 0-8014-1319-2. http://darwin-online.org.uk/content/frameset?itemID=F373&viewtype=text&pageseq=16.
Earlier related ideas were acknowledged in Darwin, Charles (1861). On the Origin of Species (3rd ed.). London: John Murray. xiii. ISBN 0-8014-1319-2. http://darwin-online.org.uk/content/frameset?itemID=F381&viewtype=text&pageseq=20.
- ↑ Gavrilets, S (2003). "Perspective: Models of Speciation: What Have We Learned in 40 Years?". Evolution 57 (10): 2197–215. doi:10.1554/02-727. PMID 14628909.
- ↑ Wolf, YI; Rogozin, IB; Grishin, NV; Koonin, EV (2002). "Genome trees and the tree of life". Trends in Genetics 18 (9): 472–9. doi:10.1016/S0168-9525(02)02744-0. PMID 12175808.
- ↑ "The Use of Model Organisms in Instruction". University of Wisconsin: Wisconsin Outreach Research Modules. http://www.loci.wisc.edu/outreach/text/model.html. Retrieved 15 March 2008.
- ↑ "NCBI: Genes and Disease". NIH: National Center for Biotechnology Information. Archived from the original on 20 February 2007. https://web.archive.org/web/20070220074727/http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=gnd&ref=sidebar. Retrieved 15 March 2008.
- ↑ Davey Smith, G; Ebrahim, S (2003). "'Mendelian randomization': Can genetic epidemiology contribute to understanding environmental determinants of disease?". International Journal of Epidemiology 32 (1): 1–22. doi:10.1093/ije/dyg070. PMID 12689998.
- ↑ "Pharmacogenetics Fact Sheet". NIH: National Institute of General Medical Sciences. Archived from the original on 12 May 2008. https://web.archive.org/web/20080512012316/http://www.nigms.nih.gov/Initiatives/PGRN/Background/FactSheet.htm. Retrieved 15 March 2008.
- ↑ Frank, SA (2004). "Genetic predisposition to cancer – insights from population genetics". Nature Reviews Genetics 5 (10): 764–72. doi:10.1038/nrg1450. PMID 15510167.
- ↑ Human Molecular Genetics 2 (second ed.). John Wiley & Sons Inc.. 1999. Chapter 18: Cancer Genetics Script error
- ↑ Lodish et al. (2000), Chapter 7: 7.1. DNA Cloning with Plasmid Vectors
- ↑ Lodish et al. (2000), Chapter 7: 7.7. Polymerase Chain Reaction: An Alternative to Cloning
- ↑ Brown TA (2002). "Section 2, Chapter 6: 6.1. The Methodology for DNA Sequencing". Genomes 2 (2nd ed.). Oxford: Bios. ISBN 1-85996-228-9. https://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=genomes.section.6452.
- ↑ Brown (2002), Section 2, Chapter 6: 6.2. Assembly of a Contiguous DNA Sequence
- ↑ Service, RF (2006). "The race for the $1000 genome". Science 311 (5767): 1544–6. doi:10.1126/science.311.5767.1544. PMID 16543431.
- ↑ Hall, Nell (May 2007). "Advanced sequencing technologies and their wider impact in microbiology". J. Exp. Biol. 210 (Pt 9): 1518–1525. doi:10.1242/jeb.001370. PMID 17449817. Template:Open access
- ↑ Church, George M. (January 2006). "Genomes for all". Sci. Am. 294 (1): 46–54. doi:10.1038/scientificamerican0106-46. PMID 16468433. Template:Link note
- ↑ Wade, Nicholas (19 March 2015). "Scientists Seek Ban on Method of Editing the Human Genome". New York Times. https://www.nytimes.com/2015/03/20/science/biologists-call-for-halt-to-gene-editing-technique-in-humans.html. Retrieved 20 March 2015.
- ↑ Pollack, Andrew (3 March 2015). "A Powerful New Way to Edit DNA". New York Times. https://www.nytimes.com/2014/03/04/health/a-powerful-new-way-to-edit-dna.html. Retrieved 20 March 2015.
- ↑ Baltimore, David; Berg, Paul; Botchan, Dana; Charo, R. Alta; Church, George; Corn, Jacob E.; Daley, George Q.; Doudna, Jennifer A. et al. (19 March 2015). "A prudent path forward for genomic engineering and germline gene modification". Science 348: 36–8. Bibcode 2015Sci...348...36B. doi:10.1126/science.aab1028. PMC 4394183. PMID 25791083. http://www.sciencemag.org/content/early/2015/03/18/science.aab1028. Retrieved 20 March 2015.
- ↑ Lanphier, Edward; Urnov, Fyodor; Haecker, Sarah Ehlen; Werner, Michael; Smolenski, Joanna (26 March 2015). "Don't edit the human germ line". Nature 519: 410–411. Bibcode 2015Natur.519..410L. doi:10.1038/519410a. PMID 25810189. http://www.nature.com/news/don-t-edit-the-human-germ-line-1.17111. Retrieved 20 March 2015.
- ↑ Kolata, Gina (23 April 2015). "Chinese Scientists Edit Genes of Human Embryos, Raising Concerns". New York Times. https://www.nytimes.com/2015/04/24/health/chinese-scientists-edit-genes-of-human-embryos-raising-concerns.html. Retrieved 24 April 2015.
- ↑ Liang, Puping (18 April 2015). "CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes". Protein & Cell 6: 363–72. doi:10.1007/s13238-015-0153-5. PMC 4417674. PMID 25894090. https://link.springer.com/article/10.1007/s13238-015-0153-5/fulltext.html. Retrieved 24 April 2015.
- Bruce Alberts; Dennis Bray; Karen Hopkin; Alexander Johnson; Julian Lewis; Martin Raff; Keith Roberts; Peter Walter (2013). Essential Cell Biology, 4th Edition. Garland Science. ISBN 978-1-317-80627-1. https://books.google.com/books?id=Cg4WAgAAQBAJ&pg=PP1.
- Griffiths, Anthony J. F.; Miller, Jeffrey H.; Suzuki, David T. et al., eds. (2000). An Introduction to Genetic Analysis (7th ed.). New York: W. H. Freeman. ISBN 0-7167-3520-2.
- Genetics: Analysis of Genes and Genomes (6th ed.). Jones & Bartlett. 2005. ISBN 0-7637-1511-5.
- King, Robert C; Mulligan, Pamela K; Stansfield, William D (2013). A Dictionary of Genetics (8th ed.). New York: Oxford University Press. ISBN 0-1997-6644-4.
- Molecular Cell Biology (4th ed.). New York: Scientific American Books. 2000. ISBN 0-7167-3136-3.
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