In video games, a boss is a particularly large or challenging computer-controlled character who must be defeated at the end of a segment of a game, whether he/she/it be for a level, an episode, or the very end of the game itself (final boss). Bosses appear in many video games, particularly story or level-based first and third-person shooters, platform games, role-playing video games, and most shoot 'em ups. Most games feature multiple bosses, each often more difficult than the last.
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- Abby Anderson (The Last of Us)
- Abyss (Marvel vs. Capcom)
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- Ace Hardlight
- Achille (Maximo)
- Adolf Hitler in popular culture
- Agahnim
- Akuma (Street Fighter)
- Ra's al Ghul
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- Alf Layla wa-Layla (Sonic the Hedgehog)
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- Alonzo Drek
- Alpha-152
- Alucard (Castlevania)
- Count Alucard (character)
- Anarcrothe
- Android 21
- Andross (Star Fox)
- Anglersaur
- Angstrom Darkwater
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- Ansem, Seeker of Darkness
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- Antonio Guster
- Aparoid
- Apocalypse (Marvel Comics)
- Aqua (Kingdom Hearts)
- Archie (Pokémon)
- Ares (God of War)
- Arkham (Devil May Cry)
- Arkham Knight
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- Army Dillo
- Saren Arterius
- Arthur (Sakura Wars)
- Alexia Ashford
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- Asura (Asura's Wrath)
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- Axel (Kingdom Hearts)
- Ayame Fujieda
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- Baby Bowser
- Babylon Guardian
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- Bald Bull
- Baldur (God of War)
- Balrog (Street Fighter)
- Bane (DC Comics)
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- Hector Barbossa
- Bearenger
- Bearminator
- Beatrix (Final Fantasy)
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- Bebop and Rocksteady
- Bellum (The Legend of Zelda)
- Richter Belmont
- Trevor Belmont
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- Big Arm (Sonic the Hedgehog)
- Big Boss (Metal Gear)
- Billy Kane
- Bio Rex
- Biolizard
- Birdo (character)
- William Birkin
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- Black Doom (Sonic the Hedgehog)
- Black Mask (comics)
- Black Shadow
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- Blaze (Mortal Kombat)
- Blaze the Cat
- Blinky (Pac-Man)
- Blood Falcon
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- Blue (Pokémon)
- Bluey (Diddy Kong Racing)
- Book of Prophecies
- The Boss (Metal Gear)
- Boss Cass
- Bowser (Super Mario)
- Bowser Jr.
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- Braig
- Brainiac (comics)
- Brock (Pokémon)
- Kyle Broflovski
- Bruno (Pokémon)
- Bubbler (Diddy Kong Racing)
- Burgh (Pokémon)
- Byrne (The Legend of Zelda)
- Byron (Pokémon)
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- Cackletta
- Noctis Lucis Caelum
- Calamity Ganon
- Cammy
- Captain Syrup
- Captain Whisker
- Carrotia
- Eric Cartman
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- Cera (The Land Before Time)
- Chain Chomp
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- Chaos (Final Fantasy character)
- Chaos (Sonic the Hedgehog)
- Chaos 2
- Chaos 4
- Chaos 6
- Chef (South Park)
- Cheren (Pokémon)
- Cia (The Legend of Zelda)
- Circle of Nine
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- Clayface
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- Cloud Strife
- Coco Bandicoot
- Chancellor Cole
- Colossal Squid (Hungry Shark)
- Colress
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- Copperhead (DC Comics)
- Nina Cortex
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- Count Bleck
- Cranky Kong
- Crazy Hand
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- Crunch Bandicoot
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- Culex (Super Mario)
- Cyber-Akuma
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- Cynthia (Pokémon)
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- D-Fekt
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- Dante (Devil May Cry)
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- Dark Gaia
- Dark Kahn
- Dark Link
- Dark Matter (Kirby)
- Dark Pit
- Dark Queen (Battletoads)
- Dark Queen (Sonic the Hedgehog)
- Dark Samus
- Dark Star (Super Mario)
- Dark Zero
- Darkseid
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- Deadshot
- Death Busters
- Death Egg Robot
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- Deathborn
- Deathstroke
- Deimos (God of War)
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- Demise (The Legend of Zelda)
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- Deoxys
- Derek C. Simmons
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- Devil Doom
- Devilotte
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- Alcina Dimitrescu
- Dingodile
- Doctor Eggman (Sonic Boom)
- Doctor Nefarious Tropy
- Doctor Neo Cortex
- Doctor Nitrus Brio
- Doctor Wily
- Jedah Dohma
- Donkey Kong (character)
- Count Dooku
- Doctor Doom
- Dormammu
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- Dracula (Castlevania)
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- Duon